From El Calafate, travel west on Route 5 (which connects El Calafate with Rio Gallegos) you'll drive 32 km toward the intersection with route 40. At this point you take the left turn on Ruta Nacional Nº 40 and continue 78 more km to the north, until you come across La Leona roadhouse on the right hand side. On the left side of the street you will see the estancia entrance gate with the big sign on top reading: Estancia "La Estela". Enter through the gate and continue for 3km until you reach the Estancia. .
From El Calafate International Airport, you use the same directions as before (West on Route 5), and drive only 19 km on Route 5 until the intersection with Ruta Nacional Nº 40 (where you will turn left and head North).
From the north (El Chalten, San Martin Lake, etc) as you travel south on route 40, you will cross a bridge over the La Leona river, and immediately on your right side you will see the entrance to the ranch, and La Leona roadhouse up on your left.